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Development of a Quantitative Real-Time PCR Assay for Detection of Mycoplasma genitalium

1 Jul 2005

Abstract excerpt

Mycoplasma genitalium is known to cause nonchlamydial, nongonococcal urethritis in men and to be associated with pelvic inflammatory disease in women. Specific and sensitive PCR methods are needed for diagnosis of this bacterium because it is very difficult to culture from patient samples. To determine the bacterial load in patients' specimens, a quantitative real-time LightCycler PCR was developed. The...

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PMID: 16000423
DOI: 10.1128/jcm.43.7.3121-3128.2005

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