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Quantitative Detection of <i>Mycoplasma genitalium</i> from First-Pass Urine of Men with Urethritis and Asymptomatic Men by Real-Time PCR

1 Apr 2002

Abstract excerpt

We developed a TaqMan-based real-time PCR assay for quantifying Mycoplasma genitalium. This assay is able to specifically quantify concentrations of the M. genitalium 16S rRNA gene ranging from 10(7) to 10 copies/reaction. Using the TaqMan assay, we quantified the M. genitalium 16S rRNA gene in first-pass urine of men with urethritis and asymptomatic men who were positive for M. genitalium by PCR- and...

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PMID: 11923372
DOI: 10.1128/jcm.40.4.1451-1455.2002

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