Effect of Demecolcine and Nocodazole on the Efficiency of Chemically Assisted Removal of Chromosomes and the Developmental Potential of Nuclear Transferred Porcine Oocytes

Abstract excerpt

Brief treatment of metaphase II (MII) stage porcine oocytes with 0.4 microg/mL demecolcine in the presence of 0.05 M sucrose produces a membrane protrusion that contains a condensed chromosome mass. The present study examined the optimal conditions for demecolcine and nocodazole treatment in chemically assisted removal of chromosomes. When matured oocytes were treated with 0.1-0.4 microg/mL demecolcine for 60 min...

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