Article
Genetic damage following introduction of DNA in Phycomyces.
Fungal genetics and biology : FG & B - 1 Feb 2004
Obraztsova Irina N, Prados Nicolás, Holzmann Klaus, Avalos Javier, Cerdá-Olmedo Enrique
Abstract excerpt
Introduction of plasmids in Phycomyces blakesleeanus caused extensive changes in the exogenous DNA and in the resident genome. Plasmids with a bacterial gene for geneticin resistance under a Phycomyces promoter were either injected into immature sporangia or incubated with spheroplasts. An improved method produced about one viable spheroplast per cell. Colonies resistant to geneticin were rare and only about 0.1%...
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