Article
Genotyping of Flavobacterium psychrophilum using PCR-RFLP analysis.
Diseases of aquatic organisms - 24 Oct 2003
Izumi Shotaro, Aranishi Futoshi, Wakabayashi Hisatsugu
Abstract excerpt
Genetic variability among 242 strains of Flavobacterium psychrophilum was characterized using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analysis. Universal Primers GYR-1 and GYR-1R, which were designed to amplify the gyrase subunit B gene (gyrB), yielded a 1178 bp PCR product encoding gyrB and a 290 bp PCR product of anonymous DNA from all F. psychrophilum strains...
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