Article
Proteome of Haemophilus ducreyi by 2-D SDS-PAGE and mass spectrometry: strain variation, virulence, and carbohydrate expression.
Journal of proteome research - 1 Jan 2000
Scheffler N Karoline, Falick Arnold M, Hall Steven C, Ray William C, Post Deborah M, Munson Robert S, Gibson Bradford W
Abstract excerpt
We have analyzed the proteome of several strains of Haemophilus ducreyi by two-dimensional gel electrophoresis (2-DE) and mass spectrometry. Over 100 spots were analyzed from the soluble and insoluble protein fractions from the prototype strain 35000HP and 122 distinct proteins were identified. Functions of approximately 80% of the 122 proteins were deduced by identification with close homologues of Haemophilus...
Topics
- Carbohydrate Metabolism
- Carbohydrate Sequence
- Chromatography, High Pressure Liquid
- Databases, Protein
- Electrophoresis, Polyacrylamide Gel
- Gene Deletion
- Genes, Bacterial
- Genetic Variation
- Genome, Bacterial
- Haemophilus ducreyi
- Haemophilus influenzae
- Lipopolysaccharides