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Development of Real-Time RT-PCR for the Detection of Avian Influenza Virus

1 Sept 2003

Abstract excerpt

A real-time reverse transcriptase/polymerase chain reaction (RRT-PCR) assay was developed using hydrolysis probes for the detection of avian influenza virus (AIV) and the H5 and H7 subtypes. The AIV specific primers and probes were directed to regions of the AIV matrix gene that are conserved among most type A influenza viruses. The H5 and H7 primers and probes are directed to H5 and H7 hemagglutinin gene regions...

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PMID: 14575115
DOI: 10.1637/0005-2086-47.s3.1079

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