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Article

Validated RealTime reverse transcriptase PCR methods for the diagnosis and pathotyping of Eurasian H7 avian influenza viruses.

Influenza and other respiratory viruses - 1 Jul 2009

Slomka Marek J, Pavlidis Theo, Coward Vivien J, Voermans John, Koch Guus, Hanna Amanda, Banks Jill, Brown Ian H

Abstract excerpt

BACKGROUND: Avian influenza (AI) caused by H7 AI viruses (AIVs) of both low pathogenicity (LP) and high pathogenicity (HP) are notifiable poultry diseases. OBJECTIVES: Design and validate two RealTime reverse transcriptase polymerase chain reactions (RRT PCRs) for Eurasian H7 AIV detection and pathotyping. METHODS: The H7 RRT PCRs amplified within the (i) HA2 and (ii) cleavage site CS regions of the...

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Identifiers

PMID: 19627372
DOI: 10.1111/j.1750-2659.2009.00083.x
PMCID: PMC4634683

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