Article
Analysis of HIV-1 envelope mutants and pseudotyping of replication-defective HIV-1 vectors by genetic complementation.
AIDS research and human retroviruses - 1 Sept 1992
Delwart E L, Buchschacher G L, Freed E O, Panganiban A T
Abstract excerpt
Infectious HIV-1 particles containing replication-defective vectors that express the hygromycin B phosphotransferase gene were generated by transient complementation in COS-1 cells. A defective vector dependent only on trans-complementation with an env gene and a small vector containing a deletion of almost all of the trans region were used to examine pseudotyping of HIV-1 by an amphotropic murine retrovirus....
Topics
- Amino Acid Sequence
- Cell Line
- Gene Products, env
- Genetic Complementation Test
- Genetic Vectors
- Giant Cells
- HIV-1
- Molecular Sequence Data
- Mutation
- Plasmids
- Virus Replication