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Article

Development of a fluorescent nanosensor for ribose.

FEBS letters - 9 Oct 2003

Lager Ida, Fehr Marcus, Frommer Wolf B, Lalonde Sylvie

Abstract excerpt

To analyze ribose uptake and metabolism in living cells, nanosensors were engineered by flanking the Escherichia coli periplasmic ribose binding protein with two green fluorescent protein variants. Following binding of ribose, fluorescence resonance energy transfer decreased with increasing ribose concentration. Five affinity mutants were generated covering binding constants between 400 nM and 11.7 mM. Analysis...

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Identifiers

PMID: 14550551
DOI: 10.1016/s0014-5793(03)00976-1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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