Article
Rapid metabolism of glucose detected with FRET glucose nanosensors in epidermal cells and intact roots of Arabidopsis RNA-silencing mutants.
The Plant cell - 1 Sept 2006
Deuschle Karen, Chaudhuri Bhavna, Okumoto Sakiko, Lager Ida, Lalonde Sylvie, Frommer Wolf B
Abstract excerpt
Genetically encoded glucose nanosensors have been used to measure steady state glucose levels in mammalian cytosol, nuclei, and endoplasmic reticulum. Unfortunately, the same nanosensors in Arabidopsis thaliana transformants manifested transgene silencing and undetectable fluorescence resonance energy transfer changes. Expressing nanosensors in sgs3 and rdr6 transgene silencing mutants eliminated silencing and...
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