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Single-Cell Enumeration of an Uncultivated TM7 Subgroup in the Human Subgingival Crevice

1 Oct 2003

Abstract excerpt

Specific oligonucleotide hybridization conditions were established for single-cell enumeration of uncultivated TM7 and IO25 bacteria by using clones expressing heterologous 16S rRNA. In situ analysis of human subgingival crevice specimens revealed that a greater proportion of samples from sites of chronic periodontitis than from healthy sites contained TM7 subgroup IO25. In addition, IO25 bacterial cells from...

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PMID: 14532094
DOI: 10.1128/aem.69.10.6294-6298.2003

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