Article
Construction of a Helicobacter pylori genome map and demonstration of diversity at the genome level.
Journal of bacteriology - 1 Nov 1992
Taylor D E, Eaton M, Chang N, Salama S M
Abstract excerpt
Genomic DNA from 30 strains of Helicobacter pylori was subjected to pulsed-field gel electrophoresis (PFGE) after digestion with NotI and NruI. The genome sizes of the strains ranged from 1.6 to 1.73 Mb, with an average size of 1.67 Mb. By using NotI and NruI, a circular map of H. pylori UA802 (1.7 Mb) which contained three copies of 16S and 23S rRNA genes was constructed. An unusual feature of the H. pylori...
Topics
- Base Sequence
- DNA Probes
- DNA, Circular
- DNA, Ribosomal
- Deoxyribonucleases, Type II Site-Specific
- Electrophoresis, Gel, Pulsed-Field
- Genetic Variation
- Genome, Bacterial
- Helicobacter pylori
- Molecular Sequence Data