Article
Genome DNA analysis and genotyping of clinical isolates of Helicobacter pylori.
Cytobios - 1 Jan 1994
Wada S, Matsuda M, Kikuchi M, Kodama T, Takei I, Ogawa S, Takahashi S, Shingaki M, Itoh T
Abstract excerpt
The genotype of genome DNA from seventeen clinical isolates of Helicobacter pylori by pulsed-field gel electrophoresis (PFGE) and arbitrarily primed polymerase chain reaction (AP-PCR) was determined. Three restriction enzymes, Apa I, Kpn I and Not I, were found to produce distributions of DNA fragments which were useful for analysis of the chromosome-sized DNA from H. pylori NCTC11637T by PFGE. Many of the...
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