Colloidal gold immunostaining for the detection of platelet reactive allo- and autoantibodies. Use on fresh and frozen cells.

Article

Colloidal gold immunostaining for the detection of platelet reactive allo- and autoantibodies. Use on fresh and frozen cells.

Haematologica - 1 Jan 2000

Borzini P, Riva M

Abstract excerpt

BACKGROUND: Fresh washed platelets are commonly used as target cells for the detection of platelet reactive antibodies. The use of stored platelets would allow a faster execution of the tests and a rapid selection of phenotyped cells. METHODS: Glass-adherent platelet monolayers were freeze stored...

Topics

Autoantibodies
Blood Platelets
Fluorescent Antibody Technique
Freezing
Gold
Humans
Immunohistochemistry
Isoantibodies
Phenotype
Reproducibility of Results
Sensitivity and Specificity
Specimen Handling

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