Article
Stability of coliphage lambda DNA replication initiator, the lambda O protein.
Journal of molecular biology - 5 Aug 1992
Wegrzyn G, Pawlowicz A, Taylor K
Abstract excerpt
The initiator of coliphage lambda DNA replication, lambda O protein, may be detected among other 35S-labeled phage and bacterial proteins by a method based on immunoprecipitation. This method makes it possible to study lambda O proteolytic degradation in lambda plasmid-harboring or lambda phage-infected cells; it avoids ultraviolet (u.v.)-irradiation of bacteria, used for depression of host protein synthesis,...
Topics
- Bacteriophage lambda
- DNA Replication
- DNA, Viral
- Escherichia coli
- Genes, Bacterial
- Kinetics
- Methionine
- Mutation
- Plasmids
- Rec A Recombinases
- Sulfur Radioisotopes
- Time Factors
