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Cloning of complete genome sets of six dsRNA viruses using an improved cloning method for large dsRNA genes

1 Sept 2002

Abstract excerpt

Cloning full-length large (>3 kb) dsRNA genome segments from small amounts of dsRNA has thus far remained problematic. Here, a single-primer amplification sequence-independent dsRNA cloning procedure was perfected for large genes and tailored for routine use to clone complete genome sets or individual genes. Nine complete viral genome sets were amplified by PCR, namely those of two human rotaviruses, two African...

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PMID: 12185276
DOI: 10.1099/0022-1317-83-9-2215

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