Article
An Avicel-affinity site in an Avicel-digesting exocellulase from a Trichoderma viride mutant.
Bioscience, biotechnology, and biochemistry - 1 Oct 1992
Goto M, Furukawa K, Hayashida S
Abstract excerpt
A single form of exo-type cellulase (Exo I; MW, 65,000), purified from a Trichoderma viride protease-depressed mutant, HK-75, digested Avicel to cellobiose exowise, and hydrolyzed cellotriose, cellotetraose, and cellopentaose in the strict manner of splitting off by cellobiose units. Exo I, howev...
Topics
- Adsorption
- Amino Acid Sequence
- Binding Sites
- Carbohydrates
- Cellulase
- Cellulose
- Cellulose 1,4-beta-Cellobiosidase
- Endopeptidases
- Fungal Proteins
- Glycoside Hydrolases
- Hydrogen-Ion Concentration
- Hydrolysis
- Isoelectric Point
- Molecular Sequence Data
- Molecular Weight
- Mutation
- Peptide Fragments
- Temperature