Article
Identification and purification of the main components of cellulases from a mutant strain of Trichoderma viride T 100-14.
Bioresource technology - 1 Oct 2008
Zhou Jin, Wang Yong-Hong, Chu Ju, Zhuang Ying-Ping, Zhang Si-Liang, Yin Peng
Abstract excerpt
A new mutant strain of fungus Trichoderma viride T 100-14 was cultivated on 1% microcrystalline cellulose (Avicel) for 120h and the resulting culture filtrate was prepared for protein identification and purification. To identify the predominant catalytic components, cellulases were separated by an adapted two-dimensional electrophoresis technique. The apparent major spots were identified by high performance...
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