Article
Effect of medium composition on the maintenance of a recombinant plasmid in Bacillus subtilis.
Enzyme and microbial technology - 1 May 1990
Shoham Y, Demain A L
Abstract excerpt
Recombinant plasmid pCED3 [confers beta-galactosidase production (LacZ+) and kanamycin resistance (Kmr)] in Bacillus subtilis was found to be both segregationally and structurally unstable. Since many solutions to segregational instability are already available, the problem of structural instability was specifically addressed by inclusion of kanamycin in the growth media. Culture instability was found to be...
Topics
- Bacillus subtilis
- Culture Media
- Galactosidases
- Kanamycin Resistance
- Mutation
- Phenotype
- Plasmids
- beta-Galactosidase