Isolation and partial characterization of mutants with elevated lipid content in Chlorella sorokiniana and Scenedesmus obliquus.

doi:10.1016/j.jbiotec.2012.03.017

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Article

Isolation and partial characterization of mutants with elevated lipid content in Chlorella sorokiniana and Scenedesmus obliquus.

Journal of biotechnology - 30 Nov 2012

Vigeolas Hélène, Duby Francéline, Kaymak Esra, Niessen Guillaume, Motte Patrick, Franck Fabrice, Remacle Claire

Abstract excerpt

This paper describes the isolation and partial biomass characterization of high triacylglycerol (TAG) mutants of Chlorella sorokiniana and Scenedesmus obliquus, two algal species considered as potential source of biodiesel. Following UV mutagenesis, 2000 Chlorella and 2800 Scenedesmus colonies were screened with a method based on Nile Red fluorescence. Several mutants with high Nile Red fluorescence were selected...

Topics

Biofuels
Biomass
Biotechnology
Chlorella
Chlorophyll
Fatty Acids
Mutagenesis
Mutation
Oxazines
Plant Proteins
Scenedesmus
Starch
Triglycerides

Source

Open in PubMed

Identifiers

PMID: 22480533
DOI: 10.1016/j.jbiotec.2012.03.017

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