Article
Detection of a single base substitution of the gene for prothrombin Tokushima. The application of PCR-SSCP for the genetic and molecular analysis of dysprothrombinemia.
International journal of hematology - 1 Feb 1992
Iwahana H, Yoshimoto K, Shigekiyo T, Shirakami A, Saito S, Itakura M
Abstract excerpt
The genetic and molecular basis of a mutant prothrombin of 'prothrombin Tokushima' was studied by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and PCR-restriction fragment length polymorphism (PCR-RFLP) analyses. The abnormal gene was detected by altered migration...
Topics
- Amino Acid Sequence
- Base Sequence
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Polymorphism, Restriction Fragment Length
- Prothrombin