Article
Cloning and identification of a gene encoding spore cortex-lytic enzyme in Bacillus thuringiensis.
Current microbiology - 1 Apr 2007
Hu Kun, Yang Haihua, Liu Gang, Tan Huarong
Abstract excerpt
Spore cortex-lytic enzymes are essential for germination in Bacilli. A gene-encoding spore cortex-lytic enzyme designated sleB was cloned from Bacillus thuringiensis. Disruption of sleB did not affect vegetative growth of B. thuringiensis, but the fall in optical density at 600 nm in the mutant spores was much slower than in the wild type strain during spore germination induced by L-alanine. Moreover, the mutant...
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