Roles of structural domains in the morphology and surface anchoring of the tetragonal paracrystalline array of Aeromonas hydrophila. Biochemical characterization of the major structural domain.

Abstract excerpt

The tetragonally arranged S-layer of Aeromonas hydrophila contains two morphological domains. The mature S-layer protein of A. hydrophila has a subunit molecular weight of 52,000, and has been reported to contain two structural domains. Here a mutant has been isolated which produces an S-layer of subunit molecular weight 38,650 as determined by sedimentation analysis. This truncated S-protein was exported via the...

Topics

• Aeromonas hydrophila
• Amino Acids
• Bacterial Outer Membrane Proteins
• Bacterial Proteins
• Cell Fractionation
• Circular Dichroism
• DNA Transposable Elements
• Membrane Glycoproteins
• Microscopy, Electron
• Molecular Weight