Article
FlaF Is a β-Sandwich Protein that Anchors the Archaellum in the Archaeal Cell Envelope by Binding the S-Layer Protein.
Structure (London, England : 1993) - 5 May 2015
Banerjee Ankan, Tsai Chi-Lin, Chaudhury Paushali, Tripp Patrick, Arvai Andrew S, Ishida Justin P, Tainer John A, Albers Sonja-Verena
Abstract excerpt
Archaea employ the archaellum, a type IV pilus-like nanomachine, for swimming motility. In the crenarchaeon Sulfolobus acidocaldarius, the archaellum consists of seven proteins: FlaB/X/G/F/H/I/J. FlaF is conserved and essential for archaellum assembly but no FlaF structures exist. Here, we truncated the FlaF N terminus and solved 1.5-Å and 1.65-Å resolution crystal structures of this monotopic membrane protein....
Topics
- Amino Acid Sequence
- Archaeal Proteins
- Binding Sites
- Cell Membrane
- Conserved Sequence
- Crystallography, X-Ray
- Dimerization
- Membrane Glycoproteins
- Models, Molecular
- Mutation
- Protein Structure, Secondary
- Sulfolobus acidocaldarius