Article
Mutation and mapping of genes involved in production of the antibiotic TA in Myxococcus xanthus.
Antimicrobial agents and chemotherapy - 1 Oct 1992
Varon M, Fuchs N, Monosov M, Tolchinsky S, Rosenberg E
Abstract excerpt
Transposition of TnV and Tn5lac into Myxococcus xanthus yielded 8,381 kanamycin-resistant mutants that were tested for antibiotic TA production. Twenty-four of the mutants were nonproducers of TA (less than 0.4 ng/ml), and 3 produced a higher level (2.5 micrograms/ml) than the parent strain (1.5 micrograms/ml). For most of the strains, there was 100% cotransduction between kanamycin resistance and the altered TA...
Topics
- Anti-Bacterial Agents
- Chromosome Mapping
- DNA Transposable Elements
- Kanamycin Resistance
- Macrolides
- Mutagenesis
- Myxococcus
- Phenotype