Article
Construction of a derivative of Agrobacterium tumefaciens C58 that does not mutate to tetracycline resistance.
Molecular plant-microbe interactions : MPMI - 1 Jan 2001
Luo Z Q, Clemente T E, Farrand S K
Abstract excerpt
Agrobacterium tumefaciens C58 mutates to tetracycline resistance at high frequency, complicating the use of many broad-host-range cloning and binary vectors that code for resistance to this antibiotic as the selection marker. Such mutations are associated with a resistant gene unit, tetC58, that is present in the genome of this strain. By deleting the tetC58 locus, we constructed NTL4, a derivative of C58 that no...
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