Differentiation of Pathogenic and Non-Pathogenic Serotype 1 Marek's Disease Viruses (MDVs) by the Polymerase Chain Reaction Amplification of the Tandem Direct Repeats within the MDV Genome

Abstract excerpt

There are no simple, direct methods to reliably distinguish oncogenic serotype 1 Marek's disease viruses (MDVs) from their attenuated variants. The present study was an attempt to apply polymerase chain reaction (PCR) to develop a rapid and sensitive assay for the presence of the MDV genome. PCR oligos were chosen to flank the 132-base-pair tandem direct repeats in the serotype 1 MDV genome. The PCR reaction was...

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