Identification of tms-26 as an allele of the gcaD gene, which encodes N-acetylglucosamine 1-phosphate uridyltransferase in Bacillus subtilis.

Abstract excerpt

The temperature-sensitive Bacillus subtilis tms-26 mutant strain was characterized biochemically and shown to be defective in N-acetylglucosamine 1-phosphate uridyltransferase activity. At the permissive temperature (34 degrees C), the mutant strain contained about 15% of the wild-type activity of this enzyme, whereas at the nonpermissive temperature (48 degrees C), the mutant enzyme was barely detectable....

Topics

• Alleles
• Bacillus subtilis
• Genes, Bacterial
• Hot Temperature
• Kinetics
• Phosphotransferases
• Protein Denaturation
• Sugar Phosphates
• Transferases (Other Substituted Phosphate Groups)
• Uridine Diphosphate Sugars
• Uridine Monophosphate