An easy method for screening and isolating rod mutants of Bacillus subtilis.

doi:10.1007/s00253-003-1489-y

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Article

An easy method for screening and isolating rod mutants of Bacillus subtilis.

Applied microbiology and biotechnology - 1 May 2004

Cheung S-H, Lai W-P, Ng T-W, Lam M-W, Cheung H-Y

Abstract excerpt

A convenient and rapid method for screening and identifying rod mutants of Bacillus subtilis is described. At the restrictive temperature (45 degrees C), all rod mutants of B. subtilis screened lost their ability to sporulate. The morphology and colour of mutant colonies grown on sporulation agar plates differed from those of rod+ cells, which were able to sporulate even at elevated temperature. These...

Topics

Bacillus subtilis
Bacterial Proteins
Bacteriological Techniques
Cell Wall
Color
Culture Media
Mutation
Peptide Hydrolases
Spores, Bacterial
Temperature

Source

Open in PubMed

Identifiers

PMID: 14669057
DOI: 10.1007/s00253-003-1489-y

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