A conditional-lethal mutant of bacillus subtilis 168 with a thermosensitive glycerol-3-phosphate cytidylyltransferase, an enzyme specific for the synthesis of the major wall teichoic acid.

Abstract excerpt

A biochemical analysis was undertaken of thermosensitive mutants of Bacillus subtilis 168 harbouring mutations in several tag genes, involved in the synthesis of the major wall teichoic acid, poly(glycerol phosphate), poly(groP). Incorporation of a pulse of [2-3H]glycerol into whole cells, following shift to the restrictive growth temperature, was used to assess synthesis of this polymer and to seek evidence of...

Topics

• Bacillus subtilis
• Genes, Bacterial
• Genes, Lethal
• Glycerol
• Macromolecular Substances
• Mutation
• Nucleotidyltransferases
• Solubility
• Substrate Specificity
• Teichoic Acids