Ty insertions upstream and downstream of native DUR1,2 promoter elements generate different patterns of DUR1,2 expression in Saccharomyces cerevisiae.

Abstract excerpt

Expression of allantoin pathway genes is subject to induction and nitrogen catabolite repression. Two classes of cis-dominant mutations (DUR80 and DUR1,2-Oh) result in overproduction of DUR1,2 mRNA. In DUR80 mutants, DUR1,2 expression remained inducible, nitrogen catabolite repression sensitive, and unresponsive to cell ploidy, i.e., overproduction was superimposed on normal gene regulation. DUR1,2-Oh mutations,...

Topics

• Base Sequence
• Chromosome Mapping
• Cloning, Molecular
• DNA Transposable Elements
• DNA, Fungal
• Epistasis, Genetic
• Gene Expression Regulation, Fungal
• Genes, Fungal
• Molecular Sequence Data
• Mutation