Article
Reaction of ferrous cytochrome c peroxidase with dioxygen: site-directed mutagenesis provides evidence for rapid reduction of dioxygen by intramolecular electron transfer from the compound I radical site.
Biochemistry - 17 Mar 1992
Miller M A, Bandyopadhyay D, Mauro J M, Traylor T G, Kraut J
Abstract excerpt
The reaction of dioxygen with the ferrous forms of the cloned cytochrome c peroxidase [CCP(MI)] and mutants of CCP(MI) prepared by site-directed mutagenesis was studied by photolysis of the respective ferrous-CO complexes in the presence of dioxygen. Reaction of ferrous CCP(MI) with dioxygen transiently formed a FeII-O2 complex (bimolecular rate constant = (3.8 +/- 0.3) x 10(4) M-1 s-1 at pH 6.0; 23 degrees C)...
Topics
- Cross-Linking Reagents
- Cytochrome-c Peroxidase
- Electron Spin Resonance Spectroscopy
- Electrons
- Electrophoresis, Polyacrylamide Gel
- Free Radicals
- Mutagenesis, Site-Directed
- Mutation
- Oxidation-Reduction