Article
CO recombination in cytochrome c peroxidase: effect of the local heme environment on CO binding explored through site-directed mutagenesis.
Biochemistry - 20 Feb 1990
Miller M A, Coletta M, Mauro J M, Putnam L D, Farnum M F, Kraut J, Traylor T G
Abstract excerpt
CO recombination to the cloned cytochrome c peroxidase [CCP(MI)] and mutants of CCP(MI) prepared by site-directed mutagenesis was examined as a function of pH by flash photolysis. The mutants examined included distal Arg 48----Leu, Lys; proximal Asp 235----Asn; and His 181----Gly. At alkaline pH,...
Topics
- Binding Sites
- Carbon Monoxide
- Cloning, Molecular
- Cytochrome-c Peroxidase
- Escherichia coli
- Genes
- Heme
- Hydrogen-Ion Concentration
- Kinetics
- Mathematics
- Models, Theoretical
- Mutation
- Peroxidases
- Protein Binding
- Recombinant Proteins
- Spectrophotometry