Article
Relationship of the cAMP-dependent protein kinase pathway to the SNF1 protein kinase and invertase expression in Saccharomyces cerevisiae.
Genetics - 1 Jan 1992
Hubbard E J, Yang X L, Carlson M
Abstract excerpt
The SNF1 protein kinase and the associated SNF4 protein are required for release of glucose repression in Saccharomyces cerevisiae. To identify functionally related proteins, we selected genes that in multicopy suppress the raffinose growth defect of snf4 mutants. Among the nine genes recovered were two genes from the cAMP-dependent protein kinase (cAPK) pathway, MSI1 and PDE2. Increased dosage of these genes...
Topics
- Blotting, Northern
- Blotting, Southern
- Cyclic AMP
- Gene Expression Regulation, Enzymologic
- Genes, Suppressor
- Glucose
- Glycoside Hydrolases
- Mutation
- Protein Kinases
- Protein Serine-Threonine Kinases
- Restriction Mapping
