Article
Use of acetone to attain highly active and soluble DNA packaging protein Gp16 of Phi29 for ATPase assay.
Virology - 1 Aug 2003
Huang Lisa P, Guo Peixuan
Abstract excerpt
All the well-defined DNA-packaging motors of the dsDNA viruses contain one pair of nonstructural DNA-packaging enzymes. Studies on the mechanism of virus DNA packaging have been seriously hampered by their insolubility. Phi29's DNA-packaging enzyme, gp16, is also hydrophobic, insoluble, and self-...
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