Performance of standard phenotypic assays for TonB activity, as evaluated by varying the level of functional, wild-type TonB.

doi:10.1128/JB.185.16.4699-4706.2003

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Article

Performance of standard phenotypic assays for TonB activity, as evaluated by varying the level of functional, wild-type TonB.

Journal of bacteriology - 1 Aug 2003

Larsen Ray A, Chen Gregory J, Postle Kathleen

Abstract excerpt

The ability of gram-negative bacterial cells to transport cobalamin and iron-siderophore complexes and their susceptibility to killing by some bacteriophages and colicins are characteristics routinely used to assay mutations of proteins in the TonB-dependent energy transduction system. These assays vary greatly in sensitivity and are subject to perturbation by overexpression of TonB and, perhaps, other proteins...

Topics

Bacterial Proteins
Bacteriophage lambda
Colicins
Culture Media
Escherichia coli
Escherichia coli Proteins
Membrane Proteins
Phenotype
Siderophores
Vitamin B 12

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Identifiers

PMID: 12896988
DOI: 10.1128/JB.185.16.4699-4706.2003
PMCID: PMC166451

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