Article
Site-directed mutagenesis and preliminary x-ray crystallographic studies of the tabtoxin resistance protein.
Protein and peptide letters - 1 Jun 2003
Ding Yi, Li Shentao, Li Xiaofeng, Sun Fei, Liu Jinyuan, Zhao Nanming, Rao Zihe
Abstract excerpt
Tabtoxin resistance protein (TTR) is an enzyme that catalyzes the acetylation of tabtoxin rendering tabtoxin-producing pathogens tolerant to their own phytotoxins. According to the structure based detoxification mechanism of TTR, three site-directed mutants Y141F, D130N and Y141F-D130N were constructed and overexpressed in E. coli. The products were then purified and their properties were analyzed by CD and DLS....
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