Article
In vitro analysis of the sequences required for transcription of the Arabidopsis thaliana 5S rRNA genes.
The Plant journal : for cell and molecular biology - 1 Jul 2003
Cloix Catherine, Yukawa Yasushi, Tutois Sylvie, Sugiura Masahiro, Tourmente Sylvette
Abstract excerpt
In vivo, we have already shown that only two of the 5S rDNA array blocks of the Arabidopsis thaliana genome produce the mature 5S rRNAs. Deletions and point mutations were introduced in an Arabidopsis 5S rDNA-transcribed region and its 5'- and 3'-flanks in order to analyse their effects on transcription activity. In vitro transcription revealed different transcription control regions. One control region essential...
Topics
- 3' Flanking Region
- 5' Flanking Region
- Arabidopsis
- Base Sequence
- DNA-Directed DNA Polymerase
- Genes, rRNA
- Molecular Sequence Data
- Mutation
- RNA, Ribosomal, 5S
- Sequence Deletion
- Transcription, Genetic