Molecular Typing of Methicillin-Resistant Staphylococcus aureus : Can PCR Replace Pulsed-Field Gel Electrophoresis?

Abstract excerpt

Pulsed-field gel electrophoresis (PFGE) is considered the "gold standard" for molecular typing of methicillin-resistant Staphylococcus aureus (MRSA). However, the method is time-consuming and expensive, and its discriminatory power may not be necessary in outbreak situations. We used a rapid multiplex PCR-based method with published primers and compared the results with those obtained by PFGE. A total of 75...

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