Article
Rapid molecular typing of methicillin-resistant Staphylococcus aureus by PCR-RFLP.
Infection control and hospital epidemiology - 1 May 2001
Wichelhaus T A, Hunfeld K P, Böddinghaus B, Kraiczy P, Schäfer V, Brade V
Abstract excerpt
OBJECTIVE: To establish a new, rapid, and reliable genotypic fingerprinting technique for methicillin-resistant Staphylococcus aureus (MRSA) typing in routine epidemiological surveillance. DESIGN: The method is based on polymerase chain reaction (PCR) restriction fragment-length polymorphism (RFLP) following HaeII digestion of simultaneously amplified parts of the protein A gene, the coagulase gene, and the...
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