Article
A fluorescent based PCR assay for the detection and quantitation of Giardia duodenalis genotypes in mixed populations.
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases - 1 Jul 2003
Johnson Megan L, Berryman David I, Reynoldson James A, Thompson R C Andrew
Abstract excerpt
A method based on the polymerase chain reaction has been developed for differentiating between genotypically and phenotypically distinct strains of Giardia duodenalis and quantifying the amount of initial template of the different genotypes in mixed populations. The assay relies on a sequence-specific probe, labelled with two fluorescent dyes, designed to bind within the small subunit ribosomal (SSU) RNA gene....
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