C-terminal extension of phaseolin with a short methionine-rich sequence can inhibit trimerisation and result in high instability.

Abstract excerpt

In an attempt to increase the content in essential amino acids methionine and tryptophan of the trimeric storage protein phaseolin, we fused a Met- and Trp-rich sequence to the C-terminus of a phaseolin variant lacking its vacuolar sorting signal, with the aim to target the protein for secretion and accumulation into the apoplast. The fate of the mutant protein, denominated Y3, was studied in transiently...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Biological Transport
• Share to Brefeldin A
• Share to Dimerization