Article
Multilocus sequence typing has better discriminatory ability for typing Vibrio cholerae than does pulsed-field gel electrophoresis and provides a measure of phylogenetic relatedness.
Journal of clinical microbiology - 1 May 2003
Kotetishvili Mamuka, Stine O Colin, Chen Yuansha, Kreger Arnold, Sulakvelidze Alexander, Sozhamannan Shanmuga, Morris J Glenn
Abstract excerpt
Twenty-two Vibrio cholerae isolates, including some from "epidemic" (O1 and O139) and "nonepidemic" serogroups, were characterized by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) by using three housekeeping genes, gyrB, pgm, and recA; sequence data were also obtained for the virulence-associated genes tcpA, ctxA, and ctxB. Even with the small number of loci used, MLST had better...
Topics
- Alleles
- Bacterial Typing Techniques
- Base Sequence
- Cholera
- DNA Primers
- DNA, Bacterial
- Disease Outbreaks
- Electrophoresis, Gel, Pulsed-Field
- Genes, Bacterial
- Genetic Variation
- Humans
- Molecular Sequence Data