Article
Targeted gene disruption of the neuronal calcium sensor 1 homologue in rice blast fungus, Magnaporthe grisea.
Bioscience, biotechnology, and biochemistry - 1 Mar 2003
Saitoh Ken-ichiro, Arie Tsutomu, Teraoka Tohru, Yamaguchi Isamu, Kamakura Takashi
Abstract excerpt
We isolated a neuronal calcium sensor 1/frequenin-like gene, Mg-NCS-1, from Magnaporthe grisea and evaluated the phenotypes of null-mutants of the gene. The putative Mg-NCS-1 protein showed high similarity to the other NCS-1 proteins. The null-mutants had normal growth and pathogenicity similar to the parental strain, but their growth was suppressed in high concentrations of Ca2+ or acidic conditions.
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