Systematic functional analysis of calcium‐signalling proteins in the genome of the rice‐blast fungus,<i>Magnaporthe oryzae</i>, using a high‐throughput RNA‐silencing system

Abstract excerpt

We developed an RNA-silencing vector, pSilent-Dual1 (pSD1), with a convergent dual promoter system that provides a high-throughput platform for functional genomics research in filamentous fungi. In the pSD1 system, the target gene was designed to be transcribed as a chimeric RNA with enhanced green fluorescent protein (eGFP) RNA. This enabled us to efficiently screen the resulting transformants using GFP...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Fungal and yeast genetics research
• Share to Microbial Natural Products and Biosynthesis
• Share to Plant-Microbe Interactions and Immunity