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Quantification of Leishmania infantum DNA by a Real-Time PCR Assay with High Sensitivity

1 Nov 2004

Abstract excerpt

A real-time PCR was developed to quantify Leishmania infantum kinetoplast DNA and optimized to reach a sensitivity of 0.0125 parasites/ml of blood. In order to analyze the incidence of heterogeneity and number of minicircles, we performed comparative PCR by using the Leishmania DNA polymerase gene as a reporter. Assays performed in both promastigote and amastigote stages showed variations among different L....

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 15528722
DOI: 10.1128/jcm.42.11.5249-5255.2004

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