PCR-Restriction Fragment Length Polymorphism Analysis of the Phospholipase B ( PLB1 ) Gene for Subtyping of Cryptococcus neoformans Isolates

Abstract excerpt

Cryptococcus neoformans is a pathogenic yeast that is currently divided into three varieties, five serotypes, and eight molecular types. The following report describes the use of PCR-restriction fragment length polymorphism (RFLP) analysis of the phospholipase B gene (PLB1) as a simple tool to differentiate between C. neoformans subgroups. A PLB1 fragment, 1,970 bp, was amplified and digested with either AvaI or...

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