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Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species

1 Aug 1990

Abstract excerpt

Detailed restriction analyses of many samples often require substantial amounts of time and effort for DNA extraction, restriction digests, Southern blotting, and hybridization. We describe a novel approach that uses the polymerase chain reaction (PCR) for rapid simplified restriction typing and mapping of DNA from many different isolates. DNA fragments up to 2 kilobase pairs in length were efficiently amplified...

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Identifiers

PMID: 2376561
DOI: 10.1128/jb.172.8.4238-4246.1990

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