Article
Detection of single nucleotide polymorphism (SNP) controlling the waxy character in wheat by using a derived cleaved amplified polymorphic sequence (dCAPS) marker.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 Jun 2003
Yanagisawa T, Kiribuchi-Otobe C, Hirano H, Suzuki Y, Fujita M
Abstract excerpt
We investigated a single nucleotide polymorphism (SNP) in the Wx-D1 gene, which was found in a mutant waxy wheat, and which expressed the Wx-D1 protein (granule-bound starch synthase I) as shown by immunoblot analysis. We also assayed starch synthase activity of granule-bound proteins. Using 22 doubled-haploid (DH) lines and 172 F(5) lines derived from the wild type x the mutant, we detected SNP via a PCR-based...
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